MB Research Labs offers newly accepted h-CLAT – In Vitro Skin Sensitization Test
The h-CLAT test method is an in vitro toxicology assay that evaluates cell surface marker expression – CD86 and CD54 on THP-1 cells, a human monocytic leukemia cell line following a 24 hrs exposure to a test substance. CD86 and CD54 are surface molecules that are typical markers of Dendritric Cell(DC) activation and play an important role in T cell priming. The cell surface marker expression is measured by flow cytometry following fluorescent staining with fluorochrome-tagged antibodies. Assessment of surface markers expression at sub-cytotoxic concentrations is also asses concurrently. The relative fluorescence intensity of the surface markers are calculated by comparing results to vehicle control are calculated and used in the h-CLAT prediction model, allowing for discrimination between sensitizers and non-sensitizers.
In preparation of regulatory acceptance, MB Research has validated and been conducting the h-CLAT for almost a year in collaboration with Japanese scientists transferring technology.
For more information about this assay and our other in vitro toxicology testing capabilities: MB Research In Vitro Toxicology